Mouse Phosphorylated AKT1 Kinase ELISA kit (E03P1047) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Mouse Phosphorylated AKT1 Kinase ELISA kit (E03P1047)

E03P1047 Mouse Phosphorylated AKT1 Kinase ELISA kit

The Mouse Phosphorylated AKT1 Kinase ELISA kit can be used to identify samples from the mouse species. Phosphorylated AKT1 Kinase can also be called p-AKT.

Products

Specifications of Mouse Phosphorylated AKT1 Kinase ELISA kit

Product Information
Cat. No.	E03P1047
Product Name	Mouse Phosphorylated AKT1 Kinase ELISA kit
Species	Mouse
Product Size	48 Tests / 96 Tests
Concentration	0.5-10ng/ml
Sensitivity	0.1ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/ml	1 vial
STANDARD B (0.5mL)	0.5 ng/ml	1 vial
STANDARD C (0.5mL)	1.0 ng/ml	1 vial
STANDARD D (0.5mL)	2.5 ng/ml	1 vial
STANDARD E (0.5mL)	5.0 ng/ml	1 vial
STANDARD F (0.5mL)	10 ng/ml	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

p-AKT ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-p-AKT antibody and a p-AKT-HRP conjugate. The assay sample and buffer are incubated together with p-AKT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the p-AKT concentration since p-AKT from samples and p-AKT-HRP conjugate compete for the anti-p-AKT antibody binding site. Since the number of sites is limited, as more sites are occupied by p-AKT from the sample, fewer sites are left to bind p-AKT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The p-AKT concentration in each sample is interpolated from this standard curve.

Quality Control on Mouse Phosphorylated AKT1 Kinase ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between p-AKT and analogues was observed.