Mouse Protein C ELISA kit (E03P0141) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Mouse Protein C ELISA kit (E03P0141)

E03P0141 Mouse Protein C ELISA kit

The Mouse Protein C ELISA kit can be used to identify samples from the mouse species. Protein C can also be called PRO-C, VKDPC, PC, Vitamin K Dependent Protein C, Inactivator Of Coagulation Factors Va And VIIIa, Autoprothrombin IIA, Blood Coagulation Factor XIV, Activation peptide, ProC.

Products

Specifications of Mouse Protein C ELISA kit

Product Information
Cat. No.	E03P0141
Product Name	Mouse Protein C ELISA kit
Species	Mouse
Product Size	48 Tests / 96 Tests
Concentration	1.0-25ng/ml
Sensitivity	0.1ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/ml	1 vial
STANDARD B (0.5mL)	1.0 ng/ml	1 vial
STANDARD C (0.5mL)	2.5 ng/ml	1 vial
STANDARD D (0.5mL)	5.0 ng/ml	1 vial
STANDARD E (0.5mL)	10 ng/ml	1 vial
STANDARD F (0.5mL)	25 ng/ml	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

ProC ELISA kit uses an anti-ProC antibody and a ProC-HRP conjugate in a competitive enzyme immunoassay method. ProC-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ProC antibody binding site between ProC from samples and ProC-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ProC. Since the number of sites is limited, as more sites are occupied by ProC from the sample, fewer sites are left to bind ProC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ProC concentration in each sample is interpolated from this standard curve.

Quality Control on Mouse Protein C ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between ProC and analogues was observed.