E03P0746 Mouse Protein Kinase A ELISA kit
The Mouse Protein Kinase A ELISA kit can be used to identify samples from the mouse species. Protein Kinase A can also be called cAMP-dependent protein kinase, PKA.
Specifications of Mouse Protein Kinase A ELISA kit
Product Information | |
Cat. No. | E03P0746 |
Product Name | Mouse Protein Kinase A ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PKA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PKA antibody and a PKA-HRP conjugate. The assay sample and buffer are incubated together with PKA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PKA concentration since PKA from samples and PKA-HRP conjugate compete for the anti-PKA antibody binding site. Since the number of sites is limited, as more sites are occupied by PKA from the sample, fewer sites are left to bind PKA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PKA concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Protein Kinase A ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PKA and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Protein Kinase A ELISA kit
Citations of Mouse Protein Kinase A ELISA kit
E03P0746 has been referenced in the below publications：
Triglyceride with medium-chain fatty acids increases the activity and expression of hormone-sensitive lipase in white adipose tissue of C57BL/6J mice.
Effect of medium chain triglycerides on brown adipose tissue and its β3 adrenergic receptors in mice with obesity induced by high fat diet.
Effect of common edible oils on lipid metabolism and its mechanism in obese mice induced by a high fat diet.
Effect of medium chain triglycerides on brown adipose tissue in obese mice.
Triglyceride with medium-chain fatty acids increases the activity and expression of hormone-sensitive lipase in white adipose tissue of C57BL/6J mice.
