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  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Protein Kinase C ELISA kit (E03P0668)

E03P0668 Mouse Protein Kinase C ELISA kit

The Mouse Protein Kinase C ELISA kit can be used to identify samples from the mouse species. Protein Kinase C can also be called PKC.

Products

Specifications of Mouse Protein Kinase C ELISA kit

Product Information

Cat. No.

E03P0668

Product Name

Mouse Protein Kinase C ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

0.5-10ng/ml

Sensitivity

0.1ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml1 vial

STANDARD B (0.5mL)

0.5 ng/ml

1 vial

STANDARD C (0.5mL)

1.0 ng/ml

1 vial

STANDARD D (0.5mL)

2.5 ng/ml

1 vial

STANDARD E (0.5mL)

5.0ng/ml

1 vial

STANDARD F (0.5mL)

10 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PKC ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PKC antibody and a PKC-HRP conjugate. The assay sample and buffer are incubated together with PKC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PKC concentration since PKC from samples and PKC-HRP conjugate compete for the anti-PKC antibody binding site. Since the number of sites is limited, as more sites are occupied by PKC from the sample, fewer sites are left to bind PKC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PKC concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Protein Kinase C ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PKC and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Protein Kinase C ELISA kit

Summary of the Assay Procedure for Mouse Protein Kinase C ELISA kit

Citations of Mouse Protein Kinase C ELISA kit

E03P0668 has been referenced in the below publications:

Quercetin and lithium chloride potentiate the protective effects of carvedilol against renal ischemia-reperfusion injury in high-fructose, high-fat diet-fed Swiss albino mice independent of renal lipid signaling.

Carvedilol protects against hepatic ischemia/reperfusion injury in high-fructose/high-fat diet-fed mice: Role of G protein-coupled receptor kinase 2 and 5.

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