Mouse Pulmonary Surfactant Associated Protein D ELISA kit (E03S0170)

BlueGene Biotech Mouse Pulmonary Surfactant Associated Protein D ELISA kit (E03S0170)

E03S0170 Mouse Pulmonary Surfactant Associated Protein D ELISA kit

The Mouse Pulmonary Surfactant Associated Protein D ELISA kit can be used to identify samples from the mouse species. Pulmonary Surfactant Associated Protein D can also be called SFTPD, PSDP, PSPD, SPD.

Products

Specifications of Mouse Pulmonary Surfactant Associated Protein D ELISA kit

Product Information
Cat. No.	E03S0170
Product Name	Mouse Pulmonary Surfactant Associated Protein D ELISA kit
Species	Mouse
Product Size	48 Tests / 96 Tests
Concentration	1.0-25ng/ml
Sensitivity	0.1ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/ml	1 vial
STANDARD B (0.5mL)	1.0 ng/ml	1 vial
STANDARD C (0.5mL)	2.5 ng/ml	1 vial
STANDARD D (0.5mL)	5.0 ng/ml	1 vial
STANDARD E (0.5mL)	10 ng/ml	1 vial
STANDARD F (0.5mL)	25 ng/ml	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

SPD ELISA kit uses an anti-SPD antibody and an SPD-HRP conjugate in a competitive enzyme immunoassay method. SPD-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SPD antibody binding site between SPD from samples and SPD-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SPD. Since the number of sites is limited, as more sites are occupied by SPD from the sample, fewer sites are left to bind SPD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SPD concentration in each sample is interpolated from this standard curve.

Quality Control on Mouse Pulmonary Surfactant Associated Protein D ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between SPD and analogues was observed.