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BlueGene Biotech Mouse Reduced Glutathione ELISA kit (E03G0367)

E03G0367 Mouse Reduced Glutathione ELISA kit

The Mouse Reduced Glutathione ELISA kit can be used to identify samples from the mouse species. Reduced Glutathione can also be called L-Reduced Glutathione, GSH.

Products

Specifications of Mouse Reduced Glutathione ELISA kit

Product Information

Cat. No.

E03G0367

Product Name

Mouse Reduced Glutathione ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

GSH ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GSH antibody and an GSH-HRP conjugate. The assay sample and buffer are incubated together with GSH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GSH concentration since GSH from samples and GSH-HRP conjugate compete for the anti-GSH antibody binding site. Since the number of sites is limited, as more sites are occupied by GSH from the sample, fewer sites are left to bind GSH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GSH concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Reduced Glutathione ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between GSH and analogues was observed.


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Summary of the Assay Procedure for Mouse Reduced Glutathione ELISA kit

Summary of the Assay Procedure for Mouse Reduced Glutathione ELISA kit

Citations of Mouse Reduced Glutathione ELISA kit

E03G0367 has been referenced in the below publications:

Protective effects of apigenin and myricetin against cisplatin-induced nephrotoxicity in mice.

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