Mouse Superoxide Dismutase 2, Mitochondrial ELISA kit (E03S0144)

BlueGene Biotech Mouse Superoxide Dismutase 2, Mitochondrial ELISA kit (E03S0144)

E03S0144 Mouse Superoxide Dismutase 2, Mitochondrial ELISA kit

The Mouse Superoxide Dismutase 2, Mitochondrial ELISA kit can be used to identify samples from the mouse species. Superoxide Dismutase 2, Mitochondrial can also be called SOD2, IPOB, MNSOD, MVCD6, IPO-B, Mn-SOD, superoxide dismutase 2, mitochondrial, superoxide dismutase 2, GClnc1.

Products

Specifications of Mouse Superoxide Dismutase 2, Mitochondrial ELISA kit

Product Information
Cat. No.	E03S0144
Product Name	Mouse Superoxide Dismutase 2, Mitochondrial ELISA kit
Species	Mouse
Product Size	48 Tests / 96 Tests
Concentration	0.5-10μg/ml
Sensitivity	0.1μg/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ug/mL	1 vial
STANDARD B (0.5mL)	0.5 ug/mL	1 vial
STANDARD C (0.5mL)	1.0 ug/mL	1 vial
STANDARD D (0.5mL)	2.5 ug/mL	1 vial
STANDARD E (0.5mL)	5.0 ug/mL	1 vial
STANDARD F (0.5mL)	10 ug/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

SOD2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-SOD2 antibody and an SOD2-HRP conjugate. The assay sample and buffer are incubated together with SOD2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SOD2 concentration since SOD2 from samples and SOD2-HRP conjugate compete for the anti-SOD2 antibody binding site. Since the number of sites is limited, as more sites are occupied by SOD2 from the sample, fewer sites are left to bind SOD2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SOD2 concentration in each sample is interpolated from this standard curve.

Quality Control on Mouse Superoxide Dismutase 2, Mitochondrial ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between SOD2 and analogues was observed.