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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Syndecan 1 ELISA kit (E03S0301)

E03S0301 Mouse Syndecan 1 ELISA kit ELISA kit

The Mouse Syndecan 1 ELISA kit can be used to identify samples from the mouse species. Syndecan can also be called SDC1/CD138.

Products

Specifications of Mouse Syndecan 1 ELISA kit

Product Information

Cat. No.

E03S0301

Product Name

Mouse Syndecan 1 ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

50-1000pg/ml

Sensitivity

1.0pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/ml1 vial

STANDARD B (0.5mL)

50 pg/ml

1 vial

STANDARD C (0.5mL)

100 pg/ml

1 vial

STANDARD D (0.5mL)

250 pg/ml

1 vial

STANDARD E (0.5mL)

500 pg/ml

1 vial

STANDARD F (0.5mL)

1000 pg/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SDC1/CD138 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-SDC1/CD138 antibody and an SDC1/CD138-HRP conjugate. The assay sample and buffer are incubated together with SDC1/CD138-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SDC1/CD138 concentration since SDC1/CD138 from samples and SDC1/CD138-HRP conjugate compete for the anti-SDC1/CD138 antibody binding site. Since the number of sites is limited, as more sites are occupied by SDC1/CD138 from the sample, fewer sites are left to bind SDC1/CD138-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SDC1/CD138 concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Syndecan 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SDC1/CD138 and analogues was observed.


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Summary of the Assay Procedure for Mouse Syndecan 1 ELISA kit

Summary of the Assay Procedure for Mouse Syndecan 1 ELISA kit

Citations of Mouse Syndecan 1 ELISA kit

E03S0301 has been referenced in the below publications:

Macrophage migration inhibitory factor is critical for dengue NS1-induced endothelial glycocalyx degradation and hyperpermeability.


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