E03T0007 Mouse Testosterone ELISA kit
The Mouse Testosterone ELISA kit can be used to identify samples from the mouse species. Testosterone can also be called Testesteron, Testeronun, TESTO.
Specifications of Mouse Testosterone ELISA kit
Product Information | |
Cat. No. | E03T0007 |
Product Name | Mouse Testosterone ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25ng/ml |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/ml | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/ml | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/ml | 1 vial |
STANDARD E (0.5mL) | 10 ng/ml | 1 vial |
STANDARD F (0.5mL) | 25 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TESTO ELISA kit uses an anti-TESTO antibody and an TESTO-HRP conjugate in a competitive enzyme immunoassay method. TESTO-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TESTO antibody binding site between TESTO from samples and TESTO-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TESTO. Since the number of sites is limited, as more sites are occupied by TESTO from the sample, fewer sites are left to bind TESTO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TESTO concentration in each sample is interpolated from this standard curve. Similarly, the Mouse Osteocalcin ELISA Kit employs a competitive enzyme immunoassay method using an anti-osteocalcin antibody and an osteocalcin-HRP conjugate to measure osteocalcin levels in mouse samples, with the color intensity inversely proportional to the osteocalcin concentration. |
Quality Control on Mouse Testosterone ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TESTO and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Testosterone ELISA kit
Citations of Mouse Testosterone ELISA kit
E03T0007 has been referenced in the below publications：
Abnormalities in the Male Reproductive System After Exposure to Diesel and Biodiesel Blend.
Pulmonary exposure to cellulose nanocrystals caused deleterious effects to reproductive system in male mice.
The Roles of Testicular C-kit Positive Cells in De novo Morphogenesis of Testis.
Inhibitory activities of some traditional Chinese herbs against testosterone 5α-reductase and effects of Cacumen platycladi on hair re-growth in testosterone-treated mice.
