E03T0886 Mouse Thiobarbituric Acid Reactive Substance ELISA kit
The Mouse Thiobarbituric Acid Reactive Substance ELISA kit can be used to identify samples from the mouse species. Thiobarbituric Acid Reactive Substance can also be called TBARS.
Specifications of Mouse Thiobarbituric Acid Reactive Substance ELISA kit
Product Information | |
Cat. No. | E03T0886 |
Product Name | Mouse Thiobarbituric Acid Reactive Substance ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100ng/ml |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TBARS ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TBARS antibody and an TBARS-HRP conjugate. The assay sample and buffer are incubated together with TBARS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TBARS concentration since TBARS from samples and TBARS-HRP conjugate compete for the anti-TBARS antibody binding site. Since the number of sites is limited, as more sites are occupied by TBARS from the sample, fewer sites are left to bind TBARS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TBARS concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Thiobarbituric Acid Reactive Substance ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TBARS and analogues was observed. | |
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Summary of the Assay Procedure for Mouse Thiobarbituric Acid Reactive Substance ELISA kit
Citations of Mouse Thiobarbituric Acid Reactive Substance ELISA kit
E03T0886 has been referenced in the below publications：
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