E03T0336 Mouse Thioredoxin ELISA kit
The Mouse Thioredoxin ELISA kit can be used to identify samples from the mouse species. Thioredoxin can also be called TXN, ADF, SASP, TRDX, TRX1, ATL-derived factor, Surface-associated sulphydryl protein, Trx.
Specifications of Mouse Thioredoxin ELISA kit
Product Information | |
Cat. No. | E03T0336 |
Product Name | Mouse Thioredoxin ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000pg/ml |
Sensitivity | 1.0pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Trx ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Trx antibody and an Trx-HRP conjugate. The assay sample and buffer are incubated together with Trx-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Trx concentration since Trx from samples and Trx-HRP conjugate compete for the anti-Trx antibody binding site. Since the number of sites is limited, as more sites are occupied by Trx from the sample, fewer sites are left to bind Trx-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Trx concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Thioredoxin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Trx and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Thioredoxin ELISA kit
Citations of Mouse Thioredoxin ELISA kit
E03T0336 has been referenced in the below publications：
Influences of Green Tea with Ｒich Zn and Se from Fenggang County，Guizhou Province on Trx Content and Liver Ultrastructure of Aging Model Mice.
