E03T0059 Mouse Thrombopoietin ELISA kit
The Mouse Thrombopoietin ELISA kit can be used to identify samples from the mouse species. Thrombopoietin can also be called THPO, MGDF, MKCSF, ML, MPLLG, THCYT1, TPO, thrombopoietin.
Specifications of Mouse Thrombopoietin ELISA kit
Product Information | |
Cat. No. | E03T0059 |
Product Name | Mouse Thrombopoietin ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500pg/ml |
Sensitivity | 1.0pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TPO ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TPO antibody and a TPO-HRP conjugate. The assay sample and buffer are incubated together with TPO-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TPO concentration since TPO from samples and TPO-HRP conjugate compete for the anti-TPO antibody binding site. Since the number of sites is limited, as more sites are occupied by TPO from the sample, fewer sites are left to bind TPO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TPO concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Thrombopoietin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TPO and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Thrombopoietin ELISA kit
Citations of Mouse Thrombopoietin ELISA kit
E03T0059 has been referenced in the below publications：
Experimental study of caffeic acid combined hydrogen-rich normal saline to treat acute radiation disease of bone marrow.
