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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Total Bile Acide ELISA kit (E03T0504)

E03T0504 Mouse Total Bile Acide ELISA kit

The Mouse Total Bile Acide ELISA kit can be used to identify samples from the mouse species. Total Bile Acide can also be called TBAM.

Products

Specifications of Mouse Total Bile Acide ELISA kit

Product Information

Cat. No.

E03T0504

Product Name

Mouse Total Bile Acide ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

0.5-10umol/L

Sensitivity

0.1 umol/L

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 umol/L

1 vial

STANDARD B (0.5mL)

0.5 umol/L

1 vial

STANDARD C (0.5mL)

1.0 umol/L

1 vial

STANDARD D (0.5mL)

2.5 umol/L

1 vial

STANDARD E (0.5mL)

5.0 umol/L

1 vial

STANDARD F (0.5mL)

10 umol/L

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TBAM ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TBAM antibody and an TBAM-HRP conjugate. The assay sample and buffer are incubated together with TBAM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TBAM concentration since TBAM from samples and TBAM-HRP conjugate compete for the anti-TBAM antibody binding site. Since the number of sites is limited, as more sites are occupied by TBAM from the sample, fewer sites are left to bind TBAM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TBAM concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Total Bile Acide ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TBAM and analogues was observed.


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Summary of the Assay Procedure for Mouse Total Bile Acide ELISA kit

Summary of the Assay Procedure for Mouse Total Bile Acide ELISA kit

Citations of Mouse Total Bile Acide ELISA kit

E03T0504 has been referenced in the below publications:

Effects of medium-chain fatty acids on cholesterol metabolism and their mechanisms in mice.

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