E03T0152 Mouse Transforming Growth Factor Alpha ELISA kit
The Mouse Transforming Growth Factor Alpha ELISA kit can be used to identify samples from the mouse species. Transforming Growth Factor Alpha can also be called ETGF, TGF-A, Protransforming Growth Factor alpha, EGF-Like TGF, or TGFa.
Product Information | |
Cat. No. | E03T0152 |
Product Name | Mouse Transforming Growth Factor Alpha ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500pg/ml |
Sensitivity | 1.0pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TGFa ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TGFa antibody and an TGFa-HRP conjugate. The assay sample and buffer are incubated together with TGFa-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TGFa concentration since TGFa from samples and TGFa-HRP conjugate compete for the anti-TGFa antibody binding site. Since the number of sites is limited, as more sites are occupied by TGFa from the sample, fewer sites are left to bind TGFa-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TGFa concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TGFa and analogues was observed. | |
E03T0152 has been referenced in the below publications：
Influence of Electroacupuncture Changqiang(GV1)and Baihui(GV20)on SCN Output Signal Molecular of Mice Bearing Breast Cancer.
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