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BlueGene Biotech Mouse Transforming Growth Factor Alpha ELISA kit (E03T0152)

E03T0152 Mouse Transforming Growth Factor Alpha ELISA kit

The Mouse Transforming Growth Factor Alpha ELISA kit can be used to identify samples from the mouse species. Transforming Growth Factor Alpha can also be called ETGF, TGF-A, Protransforming Growth Factor alpha, EGF-Like TGF, or TGFa.

Products

Specifications of Mouse Transforming Growth Factor Alpha ELISA kit

Product Information

Cat. No.

E03T0152

Product Name

Mouse Transforming Growth Factor Alpha ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

100-2500pg/ml

Sensitivity

1.0pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

100 pg/mL

1 vial

STANDARD C (0.5mL)

250 pg/mL

1 vial

STANDARD D (0.5mL)

500 pg/mL

1 vial

STANDARD E (0.5mL)

1000 pg/mL

1 vial

STANDARD F (0.5mL)

2500 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TGFa ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TGFa antibody and an TGFa-HRP conjugate. The assay sample and buffer are incubated together with TGFa-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TGFa concentration since TGFa from samples and TGFa-HRP conjugate compete for the anti-TGFa antibody binding site. Since the number of sites is limited, as more sites are occupied by TGFa from the sample, fewer sites are left to bind TGFa-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TGFa concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Transforming Growth Factor Alpha ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TGFa and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Transforming Growth Factor Alpha ELISA kit

Summary of the Assay Procedure for Mouse Transforming Growth Factor Alpha ELISA kit

Citations of Mouse Transforming Growth Factor Alpha ELISA kit

E03T0152 has been referenced in the below publications:

Influence of Electroacupuncture Changqiang(GV1)and Baihui(GV20)on SCN Output Signal Molecular of Mice Bearing Breast Cancer.

 


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