E03T0133 Mouse Trefoil Factor 3, Intestinal ELISA kit
The Mouse Trefoil Factor 3, Intestinal ELISA kit can be used to identify samples from the mouse species. Trefoil Factor 3, Intestinal can also be called TFF3, ITF, P1B, TFI, trefoil factor 3.
Specifications of Mouse Trefoil Factor 3, Intestinal ELISA kit
Product Information | |
Cat. No. | E03T0133 |
Product Name | Mouse Trefoil Factor 3, Intestinal ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000pg/ml |
Sensitivity | 1.0pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TFF3 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TFF3 antibody and an TFF3-HRP conjugate. The assay sample and buffer are incubated together with TFF3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TFF3 concentration since TFF3 from samples and TFF3-HRP conjugate compete for the anti-TFF3 antibody binding site. Since the number of sites is limited, as more sites are occupied by TFF3 from the sample, fewer sites are left to bind TFF3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TFF3 concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Trefoil Factor 3, Intestinal ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TFF3 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Trefoil Factor 3, Intestinal ELISA kit
Citations of Mouse Trefoil Factor 3, Intestinal ELISA kit
E03T0133 has been referenced in the below publications：
Endometriosis Leads to an Increased Trefoil Factor 3 Concentration in the Peritoneal Cavity but Does Not Alter Systemic Levels.
