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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Trefoil Factor 3, Intestinal ELISA kit (E03T0133)

E03T0133 Mouse Trefoil Factor 3, Intestinal ELISA kit

The Mouse Trefoil Factor 3, Intestinal ELISA kit can be used to identify samples from the mouse species. Trefoil Factor 3, Intestinal can also be called  TFF3, ITF, P1B, TFI, trefoil factor 3.

Products

Specifications of Mouse Trefoil Factor 3, Intestinal ELISA kit

Product Information

Cat. No.

E03T0133

Product Name

Mouse Trefoil Factor 3, Intestinal ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

250-5000pg/ml

Sensitivity

1.0pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TFF3 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TFF3 antibody and an TFF3-HRP conjugate. The assay sample and buffer are incubated together with TFF3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TFF3 concentration since TFF3 from samples and TFF3-HRP conjugate compete for the anti-TFF3 antibody binding site. Since the number of sites is limited, as more sites are occupied by TFF3 from the sample, fewer sites are left to bind TFF3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TFF3 concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Trefoil Factor 3, Intestinal ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TFF3 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Trefoil Factor 3, Intestinal ELISA kit

Summary of the Assay Procedure for Mouse Trefoil Factor 3, Intestinal ELISA kit

Citations of Mouse Trefoil Factor 3, Intestinal ELISA kit

E03T0133 has been referenced in the below publications:

Endometriosis Leads to an Increased Trefoil Factor 3 Concentration in the Peritoneal Cavity but Does Not Alter Systemic Levels.


 


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