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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Triglyceride ELISA kit (E03T0018)

E03T0018 Mouse Triglyceride ELISA kit

The Mouse Triglyceride ELISA kit can be used to identify samples from the mouse species. Triglyceride can also be called TAG, Triacylglycerol, Triacylglyceride, TG.

Products

Specifications of Mouse Triglyceride ELISA kit

Product Information

Cat. No.

E03T0018

Product Name

Mouse Triglyceride ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

50-1000ug/ml

Sensitivity

1.0ug/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ug/ml1 vial

STANDARD B (0.5mL)

50 ug/ml

1 vial

STANDARD C (0.5mL)

100 ug/ml

1 vial

STANDARD D (0.5mL)

250 ug/ml

1 vial

STANDARD E (0.5mL)

500 ug/ml

1 vial

STANDARD F (0.5mL)

1000 ug/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TG ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TG antibody and an TG-HRP conjugate. The assay sample and buffer are incubated together with TG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TG concentration since TG from samples and TG-HRP conjugate compete for the anti-TG antibody binding site. Since the number of sites is limited, as more sites are occupied by TG from the sample, fewer sites are left to bind TG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TG concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Triglyceride ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TG and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Triglyceride ELISA kit

Summary of the Assay Procedure for Mouse Triglyceride ELISA kit

Citations of Mouse Triglyceride ELISA kit

E03T0018 has been referenced in the below publications:

Oxamate Improves Glycemic Control and Insulin Sensitivity via Inhibition of TissueLactate Production in db/db Mice.

The research on antioxidant effect and active function of Tyr-Ala di-peptide.

种具有降血脂降血糖活性的组合物及其制备方法与应用。


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