E03U0007 Mouse Ubiquitin ELISA kit
The Mouse Ubiquitin ELISA kit can be used to identify samples from the mouse species. Ubiquitin can also be called Ubiquitin B, UbB, Ubiquitous Immunopoietic Polypeptide, Polyubiquitin B, UB.
Specifications of Mouse Ubiquitin ELISA kit
Product Information | |
Cat. No. | E03U0007 |
Product Name | Mouse Ubiquitin ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500ng/mL |
Sensitivity | 1.0ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 25 ng/ml | 1 vial |
STANDARD C (0.5mL) | 50 ng/ml | 1 vial |
STANDARD D (0.5mL) | 100 ng/ml | 1 vial |
STANDARD E (0.5mL) | 250 ng/ml | 1 vial |
STANDARD F (0.5mL) | 500 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
UB ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-UB antibody and an UB-HRP conjugate. The assay sample and buffer are incubated together with UB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the UB concentration since UB from samples and UB-HRP conjugate compete for the anti-UB antibody binding site. Since the number of sites is limited, as more sites are occupied by UB from the sample, fewer sites are left to bind UB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The UB concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Ubiquitin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between UB and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Ubiquitin ELISA kit
Citations of Mouse Ubiquitin ELISA kit
E03U0007 has been referenced in the below publications：
Protection effects of free ubiquitin against radiation and its mechanisms Abstr act.
X-rav irradiation modulates the expressions of free ubiquitin in the serum,small intestine, and heart of C57 BL/6N mice.
