E03U0008 Mouse Undercarboxylated Osteocalcin ELISA kit
The Mouse Undercarboxylated Osteocalcin ELISA kit can be used to identify samples from the mouse species. Undercarboxylated Osteocalcin can also be called ucOC.
Specifications of Mouse Undercarboxylated Osteocalcin ELISA kit
Product Information | |
Cat. No. | E03U0008 |
Product Name | Mouse Undercarboxylated Osteocalcin ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100ng/ml |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ucOC ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ucOC antibody and an ucOC-HRP conjugate. The assay sample and buffer are incubated together with ucOC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ucOC concentration since ucOC from samples and ucOC-HRP conjugate compete for the anti-ucOC antibody binding site. Since the number of sites is limited, as more sites are occupied by ucOC from the sample, fewer sites are left to bind ucOC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ucOC concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Undercarboxylated Osteocalcin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ucOC and analogues was observed. | |
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Summary of the Assay Procedure for Mouse Undercarboxylated Osteocalcin ELISA kit
Citations of Mouse Undercarboxylated Osteocalcin ELISA kit
E03U0008 has been referenced in the below publications：
In vitro, vitamin K2 and 1α, 25-dihydroxy vitamin D3 combination enhance osteoblasts anabolism of diabetic mice.
