E03V0038 Mouse Vascuoar Endothelial Cell Growth Factor Receptor 1 ELISA kit
The Mouse Vascuoar Endothelial Cell Growth Factor Receptor 1 ELISA kit can be used to identify samples from the mouse species. Vascuoar Endothelial Cell Growth Factor Receptor 1 can also be called VEGFR 1, Flt1, FLT1, FLT, FLT-1, VEGFR-1, VEGFR1, fms related tyrosine kinase 1, vascular endothelial growth factor receptor 1, fms related receptor tyrosine kinase 1．
Product Information | |
Cat. No. | E03V0038 |
Product Name | Mouse Vascuoar Endothelial Cell Growth Factor Receptor 1 ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10ng/ml |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/ml | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/ml | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/ml | 1 vial |
STANDARD E (0.5mL) | 5.0ng/ml | 1 vial |
STANDARD F (0.5mL) | 10 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
VEGFR 1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-VEGFR 1 antibody and a VEGFR 1-HRP conjugate. The assay sample and buffer are incubated together with VEGFR 1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VEGFR 1 concentration since VEGFR 1 from samples and VEGFR 1-HRP conjugate compete for the anti-VEGFR 1 antibody binding site. Since the number of sites is limited, as more sites are occupied by VEGFR 1 from the sample, fewer sites are left to bind VEGFR 1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VEGFR 1 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between VEGFR 1 and analogues was observed. | |
E03V0038 has been referenced in the below publications：
Changes in Small Intestinal Motility and Related Hormones by Acupuncture Stimulation at Zusanli (ST 36) in Mice.
Related Bluegene Biotech Products
BlueGene Biotech News
