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BlueGene Biotech Mouse Von Willebrand Factor ELISA kit (E03V0001)

E03V0001 Mouse Von Willebrand Factor ELISA kit

The Mouse Von Willebrand Factor ELISA kit can be used to identify samples from the mouse species. Von Willebrand Factor can also be called F8VWF, VWD, von Willebrand antigen 2, Vwf.

Products

Specifications of Mouse Von Willebrand Factor ELISA kit

Product Information

Cat. No.

E03V0001

Product Name

Mouse Von Willebrand Factor ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

2.5-50ng/mL

Sensitivity

0.1ng/mL

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Vwf ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Vwf. Standards or samples are then added to the microtiter plate wells and Vwf if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Vwf present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for Vwf are added to each well to sandwichthe Vwf immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Vwf and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Vwf concentration in each sample is interpolated from this standard curve.


Quality Control on Mouse Von Willebrand Factor ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Vwf selectin and analogues was observed.


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Summary of the Assay Procedure for Mouse Von Willebrand Factor ELISA kit

Summary of the Assay Procedure for Mouse Von Willebrand Factor ELISA kit

Citations of Mouse Von Willebrand Factor ELISA kit

E03V0001 has been referenced in the below publications:

Experimental Study on Mild Hyperuricemia Mouse Model.

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