NE01A0503 Human Angiopoietin 2 ELISA kit
Human Angiopoietin 2 (ANG2) ELISA kit is suitable for the detection of samples from the human species. Angiopoietin 2 can also be called as AGPT2, ANGPT2, ANG2, angiopoietin 2, and ANG-2.
Specifications of Human Angiopoietin 2 ELISA kit
| Product's Information | |
| Cat. NO. | NE01A0503 |
| Product Name | Human Heparin ELISA kit |
| Species | Human |
| Product Size | 48 Tests / 96 Tests |
| Concentration | 50-1000 ng/mL |
| Sensitivity | 1.0ng/mL |
| Principal | Sandwich ELISA |
| Sample Volume | 100 ul |
| Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
| Assay Time | 90 minutes |
| Platform | Microplate Reader |
| Conjugate | HRP |
| Detection Method | Colorimetric |
| Storage | 2-8°C |
| Kit Components | ||
| Reagents | Specification | Quantity |
| Pre-Coated Microplate(Detachable) | 96 wells | 1 plate (Keep Sealed) |
| Standard (Stock Solution, 25ng/ml) | 220ul | 1 vial |
| Standard Diluent S1 | 840ul | 1 vial |
| Standard Diluent S2 | 500ul | 1 vial |
| Standard Diluent S3 | 500ul | 1 vial |
| Standard Diluent S4 | 500ul | 1 vial |
| Standard Diluent S5 | 500ul | 1 vial |
| Standard Diluent S6 | 500ul | 1 vial |
| Standard Diluent S7 | 500ul | 1 vial |
| Standard Diluent S8 | 500ul | 1 vial |
| Detection antibody(50×) | 270ul | 1 vial |
| Detection antibody Diluent | 11ml | 1 vial |
| HRP-Streptavidin (150×) | 100ul | 1 vial |
| HRP-Streptavidin Diluent | 10.5ml | 1 vial |
| TMB Substrates | 11ml | 1 vial (Avoid Light) |
| Stop Solution | 6ml | 1 vial |
| Wash Solution (100×) | 10ml | 1 vial |
| Sample's Diluent | 10ml | 2 vials |
| Instruction Manual | 1 | |
| Principle of the Assay |
| ANG2 ELISA kit applies the solid phase sandwich enzyme immunoassay technique. The microplate has been pre-coated with antibody specific to ANG2. Standards or samples are then added to the microplate wells and ANG2 if present, will bind to the antibody pre-coated wells. Then a biotin-conjugateddetection antibody specific toANG2 is added to the wells to form a pre-coatedantibody-antigen-detection antibody Complex. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns to blue after oxidating by HRP, and finally turns to yellow after adding the stop solution. Color develops in proportion to the amount of ANG2 bound in theinitial steps. The color is measured spectrophotometrically at a wavelength of 450nm. The concentration of ANG2 in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
Quality Control on Human Angiopoietin 2 ELISA kit
| Coefficient of Variance | Intra Variation%: 5.7-9.5 | |
| Inter Variation%: 4.0-4.8 | ||
| Recovery | 85-113.77%（serum） | |
| Linearity | Diluent Ratio | Range % |
| 1:02 | 100.05-100.87 | |
| 1:04 | 97.68-99.62 | |
| 1:08 | 101.51-103.14 | |
| 1:16 | 97.28-104.00 | |
| 1:32 | 89.97-100.88 | |
| Specificity/ Cross-reactivity | Sample | Cross-Reactivity（%） |
| IL-6 | --- | |
| CCL3 | --- | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Angiopoietin 2 ELISA kit
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