Name: Human Chemokine (C-C motif) Ligand 18 ELISA kit (NE01C0064)
Brand: Shanghai BlueGene Biotech CO., LTD.
Price: 100 USD

BlueGene Biotech Human Chemokine (C-C motif) Ligand 18 ELISA kit (NE01C0064)

Immunology

NE01C0064 Human Chemokine (C-C motif) Ligand 18 ELISA Kit

Human Chemokine (C-C motif) ligand 18 ELISA kit is suitable for the detection of samples from human species. Chemokine (C-C motif) ligand 18 can also be called CCL18, chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated), AMAC-1, AMAC1, CKb7, DC-CK1, DCCK1, MIP-4, PARC, SCYA18, C-C motif chemokine ligand 18.

Products

Specifications of Human Chemokine (C-C motif) Ligand 18 ELISA kit

Product Information
Cat. NO.	NE01C0064
Products Name	Human Chemokine (C-C motif) ligand 18 ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	31.25-2000 pg/ml
Sensitivity	4.02pg/ml
Principal	Sandwich ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, and tissue homogenates
Assay Time	3 hours and 45 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
Reagents	Specification	Quantity
Pre-Coated Microplate (Detachable)	96 wells	1 plate (Keep Sealed)
Standard (Stock Solution, 20ng/ml)	130ul	1 vial
Standard Diluent S1	900ul	1 vial
Standard Diluent S2	500ul	1 vial
Standard Diluent S3	500ul	1 vial
Standard Diluent S4	500ul	1 vial
Standard Diluent S5	500ul	1 vial
Standard Diluent S6	500ul	1 vial
Standard Diluent S7	500ul	1 vial
Standard Diluent S8	500ul	1 vial
Detection antibody(100×)	140ul	1 vial
Detection antibody Diluent	10.5ml	1 vial
HRP-Streptavidin (150×)	100ul	1 vial
HRP-Streptavidin Diluent	10.5ml	1 vial
TMB Substrates	10.5ml	1 vial (Avoid Light)
Stop Solution	6ml	1 vial
Wash Solution (100×)	10ml	1 vial
Plate Sealer		4 pieces
Instruction Manual		1

Principle of the Assay

CCL18 ELISA kit applies the solid phase sandwich enzyme immunoassay technique. The microplate has been pre-coated with antibody specific to CCL18. Standards or samples are then added to the microplate wells and CCL18 if present, will bind to the antibody pre-coated wells. Then a biotin-conjugated detection antibody specific to CCL18 is added to the wells to form a pre-coatedantibody-antigen-detection antibody Complex. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns to blue after oxidating by HRP, and finally turns to yellow after adding the stop solution. Color develops in proportion to the amount of CCL18 bound in theinitial steps. The color is measured spectrophotometrically at a wavelength of 450nm. The concentration of CCL18 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Quality Control on Human Chemokine (C-C motif) Ligand 18 ELISA kit

Coefficient of Variance	Intra Variation: 4.16-5.05%
Coefficient of Variance	Inter Variation: 4.1-8.2%
Recovery	95-107%(serum)
Linearity	Diluent Ratio	Range %
	1:02	103-120
	1:04	98-112
	1:08	101-110
	1:16	96-100
	1:32	103-122
	1:64	100-105
Specificity/ Cross-reactivity	Sample	Cross-Reactivity（%）
Specificity/ Cross-reactivity	CCL3	---