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BlueGene Biotech Human/Canine/Porcine Insulin ELISA kit (NE01I0004)

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NE01I0004 Human/Canine/Porcine Insulin ELISA kit

Human/Canine/Porcine Insulin ELISA kit is suitable for the detection of samples from human species. Insulin can also be called ILPR, Insulin A chain, Insulin B chain, IRDN, INS, IDDM, IDDM1, IDDM2, ILPR, MODY10, insulin, PNDM4.

Products

Specifications of Human/Canine/Porcine Insulin ELISA kit

Product Information

Cat. NO.

NE01I0004

Product Name

Human/Canine/Porcine Insulin ELISA kit

Species

Human/Canine/Porcine

Product Size

48 Tests / 96 Tests

Concentration

7.81-500 pmol/L

Sensitivity

6.1pmol/L

Principal

Sandwich ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, and tissue homogenates

Assay Time

2 hours and 45 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

Reagents

Specification

Quantity

Pre-Coated Microplate (Detachable)

96 wells

1 plate (Keep Sealed)

Standard (Stock Solution, 5000pmol/L)

150ul

1 vial

Standard Diluent S1

900ul

1 vial

Standard Diluent S2

500ul

1 vial

Standard Diluent S3

500ul

1 vial

Standard Diluent S4

500ul

1 vial

Standard Diluent S5

500ul

1 vial

Standard Diluent S6

500ul

1 vial

Standard Diluent S7

500ul

1 vial

Standard Diluent S8500ul1 vial

Detection antibody(60×)

150ul

1 vial

Detection antibody Diluent

6ml

1 vial

HRP-Streptavidin (150×)

100ul

1 vial

HRP-Streptavidin Diluent

10.5ml

1 vial

TMB Substrates

11ml

1 vial (Avoid Light)

Stop Solution

6ml

1 vial

Wash Solution (100×)

10ml

1 vial

Plate Sealer


4 pieces

Instruction Manual


1



Principle of the Assay

This ELISA kit is applied to the solid phase sandwich Enzyme Linked Immunosorbent Assay. The microtiter plate has been pre-coated with capture antibody. The antigen to be measured and the biotin-conjugated detection antibody are then added to the microtiter plate wells sequentially to form a capture antibody-antigen-detection antibody complex. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP, and finally turns to yellow immediately after adding the stop solution. Color of TMB substrate positively correlated with total insulin bound in the initial steps. The color is measured by spectrophotometrically with wavelength of 450nm. The concentration of insulin in samples is then determined by comparing the O.D. of the samples to the standard curve.


Quality Control on Human/Canine/Porcine Insulin ELISA kit

Coefficient of Variance

Intra Variation: 3.01-6.35%

Inter Variation: 4.3-13%

Recovery

80-120%(serum)

Linearity

Diluent Ratio

Range %

1:02

94-110

1:04

98-105

1:08

98-103

1:16

97-106

1:32

96-102

1:64

98-103

Specificity/

Cross-reactivity

Sample

Cross Reactivity(%)

TfR

---

IL-8

---

FGF

---

IL-6

---


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human/Canine/Porcine Insulin ELISA kit

Summary of the Assay Procedure for Human/Canine/Porcine Insulin ELISA kit

Citataions of Human/Canine/Porcine Insulin ELISA kit

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