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BlueGene Biotech Human Leptin ELISA kit (NE01L0038)

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NE01L0038 Human Leptin ELISA kit

Human Leptin ELISA kit is suitable for the detection of samples from human species. Leptin can also be called LEP, LEPD, OB, OBS, leptin.

Products

Specifications of NE01L0038 Human Leptin ELISA kit

Product Information

Cat. NO.

NE01L0038

Product Name

Human Leptin ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

23.4-1500pg/ml

Sensitivity

13.2pg/ml

Principal

Sandwich ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, and tissue homogenates

Assay Time

3 hours and 45 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

Reagents

Specification

Quantity

Pre-Coated Microplate (Detachable)

96 wells

1 plate (Keep Sealed)

Standard (Stock Solution, 15ng/ml)

130ul

1 vial

Standard Diluent S1

900ul

1 vial

Standard Diluent S2

500ul

1 vial

Standard Diluent S3

500ul

1 vial

Standard Diluent S4

500ul

1 vial

Standard Diluent S5

500ul

1 vial

Standard Diluent S6

500ul

1 vial

Standard Diluent S7

500ul

1 vial

Standard Diluent S8500ul1 vial

Detection antibody(150×)

100ul

1 vial

Detection antibody Diluent

10.5ml

1 vial

HRP-Streptavidin (150×)

100ul

1 vial

HRP-Streptavidin Diluent

10.5ml

1 vial

TMB Substrates

10.5ml

1 vial (Avoid Light)

Stop Solution

6ml

1 vial

Wash Solution (100×)

10ml

1 vial

Plate Sealer


4 pieces

Instruction Manual


1


Principle of the Assay

Leptin ELISA kit applies the solid phase sandwich enzyme immunoassay technique. The microplate has been pre-coated with antibody specific to Leptin. Standards or samples are then added to the microplate wells and Leptin if present, will bind to the antibody pre-coated wells. Then a biotin-conjugated detection antibody specific to Leptin is added to the wells to form a pre-coatedantibody-antigen-detection antibody Complex. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns to blue after oxidating by HRP, and finally turns to yellow after adding the stop solution. Color develops in proportion to the amount of Leptin bound in theinitial steps. The color is measured spectrophotometrically at a wavelength of 450nm. The concentration of Leptin in the samples is then determined by comparing the O.D. of the samples to the standard curve.


Quality Control on Human Leptin ELISA kit

Coefficient of Variance

Intra Variation: 1.61-4.0%

Inter Variation: 2.4-5.8%

Recovery

111-120% (serum)

Linearity

Diluent Ratio

Range %

1:02

104-106

1:04

98-100

1:08

99-100

1:16

99-106

1:32

99-111

1:64

90-101


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Leptin ELISA kit

Summary of the Assay Procedure for Human Leptin ELISA kit

Citations of Human Leptin ELISA kit

Not available

Product: Not available

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