NS-D050T NS0 Host Cell DNA Residue Detection kit
The NS0 HCD Residue Detection Kit can be used for Quantitative analysis of DNA residue in recombinant protein expressed products, purified intermediate and finished products from host cells.
Specifications of NS0 Host Cell DNA Residue Detection Kit
Introduction |
This kit adopts Taqman probe fluorescence qPCR method. The kit has the advantages of high specificity and sensitivity by using specific primers & probes, LOQ can reach 3fg/μL level. The preparation process of DNA Control is completely consistent with National Standard, therefore it has high purity and no protein and ion interference. DNA Control has been calibrated by National Standard to ensure the accuracy of the sample quantitative detection. The kit provides DNA Dilution Buffer, which enables good replicate parallelism in a single experiment and good reproducibility between multiple experiments. Purchasing this kit comes with a complimentary Magnetic Residual DNA Sample Preparation Kit-50T, designed for isolating residual DNA: CG-DP050. |
| Kit Components | ||
DNA Amplification | ||
Components NO. | Components Name | Cat#/Size NS-D050T(50T) |
B1 | 2XqPCR Mix | 0.625mL |
B2 | Primer&Probe Mix | 100μL |
B3 | 2×1.5mL | |
B4 | DNA Control (30ng/μL) | 25μL |
B5 | RNase-Free H2O | 0.5mL |
B6 | 50X ROX Reference Dye(Optional) | 0.15ml |
Shipping and Storage | |
| 1 | All components are shipped on dry ice. |
| 2 | The kit should be stored at -20℃ and it is recommended to be used within one year. B2 should be stored protected from light. |
| 3 | B2/B3/B4 can be stored at -20℃ for 2 years, while B1/B5/B6 can be stored at -20℃ for 1 year. B1/B5/B6 can also be purchased together as a separate set. |
Quality Control on NS0 Host Cell DNA Residue Detection Kit
Accuracy (Standard: ≦30%) | Intra Variation% 3-11 |
Inter Variation% 4.0-8.3 | |
Recovery% | 86.1-99.6 |
| Limit of Quantitation | 3 fg/μL |
Specificity | - |
BlueGene Biotech Product Show
PCR Reaction System on NS0 Host Cell DNA Residue Detection kit
Components | Volume(μL) |
2XqPCR Mix | 12.5 |
Primer&Probe Mix | 2 |
DNA template (control or sample) | 5 |
Add water | 5.5 |
Total Volume | 25 |
Mix solution = (number of reaction wells+4) * (12.5+2+5.5)μL (including the volume lost in the 4 wells).
The detection range of the standard curve mentioned above is suitable for most experiments and can be adjusted as needed.
Criteria for Results
Standard Curve: R ²> 0.99; Amplification Efficiency: 90% ≤ Eff% ≤ 110%; Slope: -3.8~-3.1.
The recovery rate of spiked samples=(measured value of spiked samples - measured value of samples)/theoretical value of spiked samples * 100%, with a range of 50% -150%.
No Template Control (NTC): In the reaction system, replacing the target template with DNA Dilution Buffer while keeping other components unchanged, and the Ct value obtained should be 'Undetermined' or Ct value≥35.
