E07B0394 Porcine Bone Morphogenetic Protein 15 ELISA kit
The Porcine Bone Morphogenetic Protein 15 ELISA kit can be used to identify samples from the porcine species. Bone Morphogenetic Protein 15 can also be called BMP15, BMP-15, GDF9B, GDF-9B, ODG2, POF4, bone morphogenetic protein 15, Growth/differentiation factor 9B .
Product Information | |
Cat. No. | E07B0394 |
Product Name | Porcine Bone Morphogenetic Protein 15 ELISA kit |
Species | Porcine |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500pg/ml |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
BMP15 ELISA kit uses an anti-BMP15 antibody and an BMP15-HRP conjugate in a competitive enzyme immunoassay method. BMP15-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-BMP15 antibody binding site between BMP15 from samples and BMP15-HRP conjugate, the intensity of the color is inversely proportional to the concentration of BMP15. Since the number of sites is limited, as more sites are occupied by BMP15 from the sample, fewer sites are left to bind BMP15-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BMP15 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between BMP15 and analogues was observed. | |
E07B0394 has been referenced in the below publications：
An essential role for the intra- oocyte MAPK activity in the NSN- to-SN transition of germinal vesicle chromatin configuration in porcine oocytes.
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