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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Porcine Bone Morphogenetic Protein 15 ELISA kit (E07B0394)

E07B0394  Porcine Bone Morphogenetic Protein 15 ELISA kit

The Porcine Bone Morphogenetic Protein 15 ELISA kit can be used to identify samples from the porcine species. Bone Morphogenetic Protein 15 can also be called BMP15, BMP-15, GDF9B, GDF-9B, ODG2, POF4, bone morphogenetic protein 15, Growth/differentiation factor 9B .

Products

Specifications of Porcine Bone Morphogenetic Protein 15 ELISA kit

Product Information

Cat. No.

E07B0394 

Product Name

Porcine Bone Morphogenetic Protein 15 ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

100-2500pg/ml

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

100 pg/mL

1 vial

STANDARD C (0.5mL)

250 pg/mL

1 vial

STANDARD D (0.5mL)

500 pg/mL

1 vial

STANDARD E (0.5mL)

1000 pg/mL

1 vial

STANDARD F (0.5mL)

2500 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

BMP15 ELISA kit uses an anti-BMP15 antibody and an BMP15-HRP conjugate in a competitive enzyme immunoassay method. BMP15-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-BMP15 antibody binding site between BMP15 from samples and BMP15-HRP conjugate, the intensity of the color is inversely proportional to the concentration of BMP15. Since the number of sites is limited, as more sites are occupied by BMP15 from the sample, fewer sites are left to bind BMP15-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BMP15 concentration in each sample is interpolated from this standard curve.


Quality Control on Porcine Bone Morphogenetic Protein 15 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between BMP15 and analogues was observed.


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Summary of the Assay Procedure for Porcine Bone Morphogenetic Protein 15 ELISA kit

Summary of the Assay Procedure for Porcine Bone Morphogenetic Protein 15 ELISA kit

Citations of Porcine Bone Morphogenetic Protein 15 ELISA kit

E07B0394  has been referenced in the below publications:

An essential role for the intra- oocyte MAPK activity in the NSN- to-SN transition of germinal vesicle chromatin configuration in porcine oocytes.

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