Porcine cyclic Adenosine Monophosphate ELISA kit (E07C0027)

BlueGene Biotech Porcine cyclic Adenosine Monophosphate ELISA kit (E07C0027)

E07C0027 Porcine cyclic Adenosine Monophosphate ELISA kit

The Porcine cyclic Adenosine Monophosphate ELISA kit can be used to identify samples from the porcine species. cyclic Adenosine Monophosphate can also be called cAMP, cyclic AMP, or 3',5'-cyclic adenosine monophosphate, Adenosine 3′,5′-(hydrogen phosphate).

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Specifications of Porcine cyclic Adenosine Monophosphate ELISA kit

Product Information
Cat. No.	E07C0027
Product Name	Porcine cyclic Adenosine Monophosphate ELISA kit
Species	Porcine
Product Size	48 Tests / 96 Tests
Concentration	50-1000 pmol/mL
Sensitivity	1.0 pmol/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pmol/mL	1 vial
STANDARD B (0.5mL)	50 pmol/mL	1 vial
STANDARD C (0.5mL)	100 pmol/mL	1 vial
STANDARD D (0.5mL)	250 pmol/mL	1 vial
STANDARD E (0.5mL)	500 pmol/mL	1 vial
STANDARD F (0.5mL)	1000 pmol/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

cAMP ELISA kit uses an anti-cAMP antibody and an cAMP-HRP conjugate in a competitive enzyme immunoassay method. cAMP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-cAMP antibody binding site between cAMP from samples and cAMP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of cAMP. Since the number of sites is limited, as more sites are occupied by cAMP from the sample, fewer sites are left to bind cAMP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The cAMP concentration in each sample is interpolated from this standard curve.

Quality Control on Porcine cyclic Adenosine Monophosphate ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between cAMP and analogues was observed.