E07G0101 Porcine Glucagon ELISA kit
The Porcine Glucagon ELISA kit can be used to identify samples from the porcine species. Glucagon can also be called GC, GCG, GLP1, GLP2, GRPP, glucagon.
Specifications of Porcine Glucagon ELISA kit
Product Information | |
Cat. No. | E07G0101 |
Product Name | Porcine Glucagon ELISA kit |
Species | Porcine |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/ml |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2..5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
GC ELISA kit uses an anti-GC antibody and an GC-HRP conjugate in a competitive enzyme immunoassay method. GC-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GC antibody binding site between GC from samples and GC-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GC. Since the number of sites is limited, as more sites are occupied by GC from the sample, fewer sites are left to bind GC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GC concentration in each sample is interpolated from this standard curve. |
Quality Control on Porcine Glucagon ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between GC and analogues was observed. | |
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Summary of the Assay Procedure for Porcine Glucagon ELISA kit
Citations of Porcine Glucagon ELISA kit
E07G0101 has been referenced in the below publications：
Correlation between three glycometabolic-related hormones and muscle glycolysis, as well as meat quality, in three pig breeds.
Meat and nutritional quality comparison of purebred and crossbred pigs.
