E07G0234 Porcine Glutathione Peroxidase 1 ELISA kit
The Porcine Glutathione Peroxidase 1 ELISA kit can be used to identify samples from the porcine species. Glutathione Peroxidase 1 can also be called GPX1, GSHPX1, Cellular glutathione peroxidase.
Specifications of Porcine Glutathione Peroxidase 1 ELISA kit
Product Information | |
Cat. No. | E07G0234 |
Product Name | Porcine Glutathione Peroxidase 1 ELISA kit |
Species | Porcine |
Product Size | 48 Tests / 96 Tests |
Concentration | 5-100 ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
GPX1 ELISA kit uses an anti-GPX1 antibody and an GPX1-HRP conjugate in a competitive enzyme immunoassay method. GPX1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GPX1 antibody binding site between GPX1 from samples and GPX1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GPX1. Since the number of sites is limited, as more sites are occupied by GPX1 from the sample, fewer sites are left to bind GPX1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPX1 concentration in each sample is interpolated from this standard curve. |
Quality Control on Porcine Glutathione Peroxidase 1 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between GPX1 and analogues was observed. | |
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Summary of the Assay Procedure for Porcine Glutathione Peroxidase 1 ELISA kit
Citations of Porcine Glutathione Peroxidase 1 ELISA kit
E07G0234 has been referenced in the below publications：
Dietary supplementation with L-glutamate and L-aspartate alleviates oxidative stress in weaned piglets challenged with hydrogen peroxide.
