E07G0023 Porcine Glycogen ELISA kit
The Porcine Glycogen ELISA kit can be used to identify samples from the porcine species. Glycogen can also be called Glycogen from oyster Type Ⅱ, Glycogen from oyster, Gly.
Specifications of Porcine Glycogen ELISA kit
Product Information | |
Cat. No. | E07G0023 |
Product Name | Porcine Glycogen ELISA kit |
Species | Porcine |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ug/mL |
Sensitivity | 0.1 ug/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ug/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ug/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ug/mL | 1 vial |
STANDARD E (0.5mL) | 10 ug/mL | 1 vial |
STANDARD F (0.5mL) | 25 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Glycogen ELISA kit uses an anti-Glycogen antibody and an Glycogen-HRP conjugate in a competitive enzyme immunoassay method. Glycogen-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Glycogen antibody binding site between Glycogen from samples and Glycogen-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Glycogen. Since the number of sites is limited, as more sites are occupied by Glycogen from the sample, fewer sites are left to bind Glycogen-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Glycogen concentration in each sample is interpolated from this standard curve. |
Quality Control on Porcine Glycogen ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Glycogen and analogues was observed. | |
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Summary of the Assay Procedure for Porcine Glycogen ELISA kit
Citations of Porcine Glycogen ELISA kit
E07G0023 has been referenced in the below publications：
Comprehensive Analysis of lncRNAs and circRNAs Reveals the Metabolic Specialization in Oxidative and Glycolytic Skeletal Muscles.
