Porcine High Mobility Group box1 Protein ELISA kit (E07H0009)

BlueGene Biotech Porcine High Mobility Group box1 Protein ELISA kit (E07H0009)

Epigenetics and Nuclear Signaling

E07H0009 Porcine High Mobility Group box1 Protein ELISA kit

The Porcine High Mobility Group box1 Protein ELISA kit can be used to identify samples from the porcine species. High Mobility Group box1 Protein can also be called HMGB1, HMG1, HMG3, SBP1, Sulfoglucuronyl Carbohydrate Binding Protein, Amphoterin, High Mobility Group Box 1 Protein.

Products

Specifications of Porcine High Mobility Group box1 Protein ELISA kit

Product Information
Cat. No.	E07H0009
Product Name	Porcine High Mobility Group box1 Protein ELISA kit
Species	Porcine
Product Size	48 Tests / 96 Tests
Concentration	5-100 ng/mL
Sensitivity	1.0 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	5 ng/mL	1 vial
STANDARD C (0.5mL)	10 ng/mL	1 vial
STANDARD D (0.5mL)	25 ng/mL	1 vial
STANDARD E (0.5mL)	50 ng/mL	1 vial
STANDARD F (0.5mL)	100 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

HMGB1 ELISA kit uses an anti-HMGB1 antibody and an HMGB1-HRP conjugate in a competitive enzyme immunoassay method. HMGB1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-HMGB1 antibody binding site between HMGB1 from samples and HMGB1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of HMGB1. Since the number of sites is limited, as more sites are occupied by HMGB1 from the sample, fewer sites are left to bind HMGB1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HMGB1 concentration in each sample is interpolated from this standard curve.

Quality Control on Porcine High Mobility Group box1 Protein ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between HMGB1 and analogues was observed.