E07H0014 Porcine Hydroxyproline ELISA kit
The Porcine Hydroxyproline ELISA kit can be used to identify samples from the porcine species. Hydroxyproline can also be called Hyp, L-hydroxyproline,(2S,4R)-4-Hydroxyproline, L-Hydroxyproline .
Specifications of Porcine Hydroxyproline ELISA kit
Product Information | |
Cat. No. | E07H0014 |
Product Name | Porcine Hydroxyproline ELISA kit |
Species | Porcine |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ug/ml |
Sensitivity | 0.1 ug/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 2..5 ug/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ug/mL | 1 vial |
STANDARD D (0.5mL) | 10 ug/mL | 1 vial |
STANDARD E (0.5mL) | 25 ug/mL | 1 vial |
STANDARD F (0.5mL) | 50 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
HYP ELISA kit uses an anti-HYP antibody and an HYP-HRP conjugate in a competitive enzyme immunoassay method. HYP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-HYP antibody binding site between HYP from samples and HYP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of HYP. Since the number of sites is limited, as more sites are occupied by HYP from the sample, fewer sites are left to bind HYP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HYP concentration in each sample is interpolated from this standard curve. |
Quality Control on Porcine Hydroxyproline ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between HYP and analogues was observed. | |
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Summary of the Assay Procedure for Porcine Hydroxyproline ELISA kit
Citations of Porcine Hydroxyproline ELISA kit
E07H0014 has been referenced in the below publications：
Impact of parity on bone metabolism throughout the reproductive cycle in sows.
Impact of Deserosalization on Small Bowel Anastomosis Healing in Swine: A Pilot Study.
