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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Porcine Insulin like Growth Factor 1 ELISA kit (E07I0009)

E07I0009 Porcine Insulin like Growth Factor 1 ELISA kit

The Porcine Insulin like Growth Factor 1 ELISA kit can be used to identify samples from the porcine species. Insulin like Growth Factor 1 can also be called IGF1, IBP1, IGF IA, IGF IB, IGFI, IGFIA, Insulin Like Growth Factor 1, Insulin like growth factor IA, Insulin like growth factor IB, Mechano growth factor, MGF, Mechano growth factor, MGF, Somatomedia C, Somatomedin C.

Products

Specifications of Porcine Insulin like Growth Factor 1 ELISA kit

Product Information

Cat. No.

E07I0009

Product Name

Porcine Insulin like Growth Factor 1 ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IGF1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IGF1. Standards or samples are then added to the microtiter plate wells and IGF1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IGF1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IGF1 are added to each well to “sandwich” the IGF1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IGF1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGF1 concentration in each sample is interpolated from this standard curve.


Quality Control on Porcine Insulin like Growth Factor 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IGF1 and analogues was observed.


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Summary of the Assay Procedure for Porcine Insulin like Growth Factor 1 ELISA kit

Summary of the Assay Procedure for Porcine Insulin like Growth Factor 1 ELISA kit

Citations of Porcine Insulin like Growth Factor 1 ELISA kit

E07I0009 has been referenced in the below publications:

Repair of porcine articular cartilage defect with autologous adipose-derived stem cells with different dose of inducing factors type I collagen scaffold.

Effects of Dietary Selenium Sources and Vitamin E Levels during  Sows’Gestation and Lactation on Organ Indices and Serum Hormone Levels of Their Progeny.


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