Porcine Interleukin 10 ELISA kit (E07I0023) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Porcine Interleukin 10 ELISA kit (E07I0023)

E07I0023 Porcine Interleukin 10 ELISA kit

The Porcine Interleukin 10 ELISA kit can be used to identify samples from the porcine species. Interleukin 10 can also be called IL 10, Interleukin-10 precursor, Cytokine synthesis inhibitory factor, CSIF, IL10A, Interleukin10, TGIF.

Products

Specifications of Porcine Interleukin 10 ELISA kit

Product Information
Cat. No.	E07I0023
Product Name	Porcine Interleukin 10 ELISA kit
Species	Porcine
Product Size	48 Tests / 96 Tests
Concentration	50-1000 pg/mL
Sensitivity	1.0 pg/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	50 pg/mL	1 vial
STANDARD C (0.5mL)	100 pg/mL	1 vial
STANDARD D (0.5mL)	250 pg/mL	1 vial
STANDARD E (0.5mL)	500 pg/mL	1 vial
STANDARD F (0.5mL)	1000 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

IL 10 ELISA kit uses an anti-IL 10 antibody and an IL 10-HRP conjugate in a competitive enzyme immunoassay method. IL 10-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IL 10 antibody binding site between IL 10 from samples and IL 10-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IL 10. Since the number of sites is limited, as more sites are occupied by IL 10 from the sample, fewer sites are left to bind IL 10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 10 concentration in each sample is interpolated from this standard curve.

Quality Control on Porcine Interleukin 10 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between IL 10 and analogues was observed.