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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Porcine Interleukin 10 ELISA kit (E07I0023)

E07I0023 Porcine Interleukin 10 ELISA kit

The Porcine Interleukin 10 ELISA kit can be used to identify samples from the porcine species. Interleukin 10 can also be called IL 10, Interleukin-10 precursor, Cytokine synthesis inhibitory factor, CSIF, IL10A, Interleukin10, TGIF.

Products

Specifications of Porcine Interleukin 10 ELISA kit

Product Information

Cat. No.

E07I0023

Product Name

Porcine Interleukin 10 ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IL 10 ELISA kit uses an anti-IL 10 antibody and an IL 10-HRP conjugate in a competitive enzyme immunoassay method. IL 10-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IL 10 antibody binding site between IL 10 from samples and IL 10-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IL 10. Since the number of sites is limited, as more sites are occupied by IL 10 from the sample, fewer sites are left to bind IL 10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 10 concentration in each sample is interpolated from this standard curve.


Quality Control on Porcine Interleukin 10 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IL 10 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Porcine Interleukin 10 ELISA kit

Summary of the Assay Procedure for Porcine Interleukin 10 ELISA kit

Citations of Porcine Interleukin 10 ELISA kit

E07I0023 has been referenced in the below publications:

Effects of feeding an herbal preparation to sows on immunological performance of offspring.

Effects of direct hemoperfusion with polyvinylidene difluoride-serine column in experimental sepsis.

蜂胶乙醇提取物对断奶仔猪血液生理及免疫功能的影响。

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