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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Porcine Interleukin 4 ELISA kit (E07I0007)

E07I0007 Porcine Interleukin 4 ELISA kit

The Porcine Interleukin 4 ELISA kit can be used to identify samples from the porcine species. Interleukin 4 can also be called IL4, BSF1, BCGF1 ,B Cell Stimulatory Factor 1, Lymphocyte stimulatory factor 1, B Cell Growth Factor, Binetrakin, Pitrakinra.

Products

Specifications of Porcine Interleukin 4 ELISA kit

Product Information

Cat. No.

E07I0007

Product Name

Porcine Interleukin 4 ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IL 4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IL 4. Standards or samples are then added to the microtiter plate wells and IL 4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IL 4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IL 4 are added to each well to “sandwich” the IL 4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL 4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 4 concentration in each sample is interpolated from this standard curve.


Quality Control on Porcine Interleukin 4 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IL 4 and analogues was observed.


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Summary of the Assay Procedure for Porcine Interleukin 4 ELISA kit

Summary of the Assay Procedure for Porcine Interleukin 4 ELISA kit

Citations of Porcine Interleukin 4 ELISA kit

E07I0007 has been referenced in the below publications:

Improved Cytotoxic T Lymphocyte Responses to Vaccination with Porcine Reproductive and Respiratory Syndrome Virus in 4-1BB Transgenic Pigs.

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