E07M0345 Porcine Metallothionein 2 ELISA kit
The Porcine Metallothionein 2 ELISA kit can be used to identify samples from the porcine species. Metallothionein 2 can also be called MT2A, MT2 Metallothionein 2A, MT-II, MT-2, MT2, MT 2, MT-2, CES1.
Specifications of Porcine Metallothionein 2 ELISA kit
Product Information | |
Cat. No. | E07M0345 |
Product Name | Porcine Metallothionein 2 ELISA kit |
Species | Porcine |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MT2 ELISA kit uses an anti-MT2 antibody and an MT2-HRP conjugate in a competitive enzyme immunoassay method. MT2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MT2 antibody binding site between MT2 from samples and MT2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MT2. Since the number of sites is limited, as more sites are occupied by MT2 from the sample, fewer sites are left to bind MT2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MT2 concentration in each sample is interpolated from this standard curve. |
Quality Control on Porcine Metallothionein 2 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MT2 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Porcine Metallothionein 2 ELISA kit
Citations of Porcine Metallothionein 2 ELISA kit
E07M0345 has been referenced in the below publications：
Metallic Orthodontic Materials Induce Gene Expression and Protein Synthesis of Metallothioneins.
