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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Porcine Metallothionein ELISA kit (E07M0030)

  • Epigenetics and Nuclear Signaling
  • Cell Biology

E07M0030 Porcine Metallothionein ELISA kit

The Porcine Metallothionein ELISA kit can be used to identify samples from the porcine species. Metallothionein can also be called CES 1, MT 2,MT 2A, MT IA, MT II, MT1.

Products

Specifications of Porcine Metallothionein ELISA kit

Product Information

Cat. No.

E07M0030

Product Name

Porcine Metallothionein ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.1 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

MT ELISA kit uses an anti-MT antibody and an MT-HRP conjugate in a competitive enzyme immunoassay method. MT-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MT antibody binding site between MT from samples and MT-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MT. Since the number of sites is limited, as more sites are occupied by MT from the sample, fewer sites are left to bind MT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MT concentration in each sample is interpolated from this standard curve.


Quality Control on Porcine Metallothionein ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between MT and analogues was observed.


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Summary of the Assay Procedure for Porcine Metallothionein ELISA kit

Summary of the Assay Procedure for Porcine Metallothionein ELISA kit

Citations of Porcine Metallothionein ELISA kit

E07M0030 has been referenced in the below publications:

No indications that zinc and protein source affect Zn bioavailability in sows during late gestation fed adequate dietary Zn concentrations.

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