E07N0021 Porcine Nuclear factor Kb p65 ELISA kit
The Porcine Nuclear factor Kb p65 ELISA kit can be used to identify samples from the porcine species. Nuclear factor Kb p65 can also be called NFkB p65, NF kB P65, NFKBp65, p65 NF kappaB, p65 NFkB, RELA, Transcription Factor p65.
Product Information | |
Cat. No. | E07N0021 |
Product Name | Porcine Nuclear factor Kb p65 ELISA kit |
Species | Porcine |
Product Size | 48 Tests / 96 Tests |
Concentration | 5-100 ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
NFkB p65 ELISA kit uses an anti-NFkB p65 antibody and an NFkB p65-HRP conjugate in a competitive enzyme immunoassay method. NFkB p65-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-NFkB p65 antibody binding site between NFkB p65 from samples and NFkB p65-HRP conjugate, the intensity of the color is inversely proportional to the concentration of NFkB p65. Since the number of sites is limited, as more sites are occupied by NFkB p65 from the sample, fewer sites are left to bind NFkB p65-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NFkB p65 concentration in each sample is interpolated from tNFkB p65 standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between NFkB p65 and analogues was observed. | |
E07N0021 has been referenced in the below publications：
The effects of baicalin on NF-kB and NLRP3 signaling pathway in piglets peripheral blood mononuclear cells subjected to immunological stress.
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