E07T0005 Porcine Transforming Growth Factor β2 ELISA kit
The Porcine Transforming Growth Factor β2 ELISA kit can be used to identify samples from the porcine species. Transforming Growth Factor β2 can also be called TGFβ2, TGF-B2, TGF-Beta2, G-TSF, LAP, Cetermin, Polyergin, Latency-associated peptide, BSC-1 cell growth inhibitor, Glioblastoma-derived T-cell suppressor factor.
Product Information | |
Cat. No. | E07T0005 |
Product Name | Porcine Transforming Growth Factor β2 ELISA kit |
Species | Porcine |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TGFβ2 ELISA kit uses an anti-TGFβ2 antibody and an TGFβ2-HRP conjugate in a competitive enzyme immunoassay method. TGFβ2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TGFβ2 antibody binding site between TGFβ2 from samples and TGFβ2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TGFβ2. Since the number of sites is limited, as more sites are occupied by TGFβ2 from the sample, fewer sites are left to bind TGFβ2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TGFβ2 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TGFβ2 and analogues was observed. | |
E07T0005 has been referenced in the below publications：
Repair of porcine articular cartilage defect with autologous adipose-derived stem cells with different dose of inducing factors type I collagen scaffold.
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