E07V0002 Porcine Vascular Cell Adhesion Molecule 1 ELISA kit
The Porcine Vascular Cell Adhesion Molecule 1 ELISA kit can be used to identify samples from the porcine species. Vascular Cell Adhesion Molecule 1 can also be called CD 106, CD106, CD106 Antigen, INCAM 100, L1CAM, Vascular Cell Adhesion Molecule 1, Vascular cell adhesion protein 1, VCAM 1, VCAM-1, INCAM-100, V-CAM 1.
Product Information | |
Cat. No. | E07V0002 |
Product Name | Porcine Vascular Cell Adhesion Molecule 1 ELISA kit |
Species | Porcine |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
VCAM 1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for VCAM 1. Standards or samples are then added to the microtiter plate wells and VCAM 1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of VCAM 1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for VCAM 1 are added to each well to “sandwich” the VCAM 1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain VCAM 1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VCAM 1 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between VCAM 1 and analogues was observed. | |
E07V0002 has been referenced in the below publications：
Effect of baicalin on the expression of adhesion molecules in porcine vascular endothelial cells induced by Haemophilus parasuis.
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