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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Porcine Vascular Cell Adhesion Molecule 1 ELISA kit (E07V0002)

E07V0002 Porcine Vascular Cell Adhesion Molecule 1 ELISA kit

The Porcine Vascular Cell Adhesion Molecule 1 ELISA kit can be used to identify samples from the porcine species. Vascular Cell Adhesion Molecule 1 can also be called CD 106, CD106, CD106 Antigen, INCAM 100, L1CAM, Vascular Cell Adhesion Molecule 1, Vascular cell adhesion protein 1, VCAM 1, VCAM-1, INCAM-100, V-CAM 1.

Products

Specifications of Porcine Vascular Cell Adhesion Molecule 1 ELISA kit

Product Information

Cat. No.

E07V0002

Product Name

Porcine Vascular Cell Adhesion Molecule 1 ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

50-1000 ng/mL

Sensitivity

1.0 ng/mL

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

50 ng/mL

1 vial

STANDARD C (0.5mL)

100 ng/mL

1 vial

STANDARD D (0.5mL)

250 ng/mL

1 vial

STANDARD E (0.5mL)

500 ng/mL

1 vial

STANDARD F (0.5mL)

1000 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

VCAM 1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for VCAM 1. Standards or samples are then added to the microtiter plate wells and VCAM 1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of VCAM 1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for VCAM 1 are added to each well to “sandwich” the VCAM 1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain VCAM 1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VCAM 1 concentration in each sample is interpolated from this standard curve.


Quality Control on Porcine Vascular Cell Adhesion Molecule 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between VCAM 1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Porcine Vascular Cell Adhesion Molecule 1 ELISA kit

Summary of the Assay Procedure for Porcine Vascular Cell Adhesion Molecule 1 ELISA kit

Citations of Porcine Vascular Cell Adhesion Molecule 1 ELISA kit

E07V0002 has been referenced in the below publications:

Effect of baicalin on the expression of adhesion molecules in porcine vascular endothelial cells induced by Haemophilus parasuis.

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