NEGES0890-48T Protein A ELISA kit
Protein A ELISA kit is suitable for the detection of samples from general species.
Specifications of Protein A ELISA kit
Product Information | |
Cat. NO. | NEGES0890-48T |
Product Name | Protein A ELISA kit |
Species | General |
Product Size | 48 Tests |
Concentration | 3.125-200 pg/ml |
Sensitivity | 1.4pg/ml |
Principal | Sandwich ELISA |
Sample Volume | 100 ul |
Sample Type | ELISA Kit for the quantitative Measurement of Protein A Residues in Protein Purification Process, and End-Product (purified fermentation broth, cell culture supernatant, etc.) |
Assay Time | 3 hours |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
Reagents | Specification | Quantity |
Pre-Coated Microplate (Detachable) | 48 wells | 1 plate (Keep Sealed) |
| Standard (Stock Solution) -2ug/ml | 10ul | 1 vial |
Standard S1 | 200pg/ml | 1 vial |
Standard S2 | 100pg/ml | 1 vial |
Standard S3 | 50pg/ml | 1 vial |
Standard S4 | 25pg/ml | 1 vial |
Standard S5 | 12.5pg/ml | 1 vial |
Standard S6 | 6.25pg/ml | 1 vial |
Standard S7 | 3.125pg/ml | 1 vial |
HRP-conjugated antibody(200×) | 40ul | 1 vial |
TMB Substrates | 10ml | 1 vial (Avoid Light) |
Stop Solution | 10ml | 1 vial |
Wash Solution (100×) | 10ml | 1 vial |
| Diluent Buffer(10x) | 10ml | 1 vial |
| Diluent Ingredient | 0.5g | 1 tube |
Plate Sealer | 4 pieces | |
Instruction Manual | 1 | |
Principle of the Assay |
This ELISA kit is applied to the double antibody sandwich Enzyme Linked Immunosorbent Assay to detect the concentration of protein A in samples. The microtiter plate has been pre-coated with chicken anti-protein A antibody , standards or samples are then added to the microtiter plate wells and protein A if present, will bind to the antibody pre-coated wells under specific conditions. After washing, then a Horseradish Peroxidase(HRP)-conjugated detection rabbit anti-protein A antibody is added to the wells, under 37℃ incubation, to form a precoated antibody-protein A-detection antibody sandwich complex. Then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP, and finally turns to yellow immediately after adding the stop solution. Color of TMB substrate positively correlated with total protein A bound in the initial steps. The color is measured by spectrophotometrically with wavelength of 450nm. The concentration of protein A in samples is then determined by comparing the O.D. of the samples to the standard curve. |
Quality Control on Protein A ELISA kit
Coefficient of Variance | Intra Variation: 4-8% | ||
Inter Variation: 8-10% | |||
Recovery | 67.36-115.92% | ||
Linearity | Diluent Ratio | Range % | Average Linearity % |
4 | 98.25-105.77 | 102.01 | |
40 | 100.68-109.74 | 105.21 | |
Specificity/ Cross-reactivity | Sample | Cross-Reactivity（%） | |
Bovine IgG | --- | ||
Goat IgG | --- | ||
Human IgG | --- | ||
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Summary of the Assay Procedure for Protein A ELISA kit
Citations of Protein A ELISA Kit
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