E04A0659 Rabbit 17 Hydroxycorticosteroids ELISA kit
Rabbit 17 Hydroxycorticosteroids ELISA kit is suitable for detecting samples from rabbit species. 17 Hydroxycorticosteroids can also be called 17 OHCSs, 17 Hydroxycorticosteroids, 17 OHCS.
Rabbit 17 Hydroxycorticosteroids ELISA kit
Product Information | |
Cat. No. | E04A0659 |
Product Name | Rabbit 17 Hydroxycorticosteroids ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10nmol/mL |
Sensitivity | 0.1 nmol/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 nmol/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 nmol/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 nmol/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 nmol/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 nmol/mL | 1 vial |
STANDARD F (0.5mL) | 10 nmol/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
17 OHCS ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- 17 OHCS antibody and an 17 OHCS-HRP conjugate. The assay sample and buffer are incubated together with 17 OHCS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 17 OHCS concentration since 17 OHCS from samples and 17 OHCS-HRP conjugate compete for the anti-17 OHCS antibody binding site. Since the number of sites is limited, as more sites are occupied by 17 OHCS from the sample, fewer sites are left to bind 17 OHCS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 17 OHCS concentration in each sample is interpolated from this standard curve. |
Quality Control on Rabbit 17 Hydroxycorticosteroids ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-114.79% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-116 | |
1:4 | 91-109 | |
1:8 | 88-103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between 17 OHCS and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rabbit 17 Hydroxycorticosteroids ELISA kit
Citations of Rabbit 17 Hydroxycorticosteroids ELISA kit
E04A0659 has been referenced in the below publications:
脾肾阳虚型IBS-D大鼠模型探索及温肾健脾方干预CRF信号通路的机制研究.
