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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit 3 Hydroxy 3 methylglutaryl CoA Elisa kit (E04H1417)

E04H1417 Rabbit 3 Hydroxy 3 methylglutaryl CoA Elisa kit

Rabbit 3 Hydroxy 3 methylglutaryl CoA Elisa kit is suitable for detecting samples from rabbit species. 3 Hydroxy 3 methylglutaryl CoA can also be called 3-hydroxy-3-methylglutaryl CoA, 3-hydroxy-3-methylglutaryl coenzyme A.

Products

Specifications of Rabbit 3 Hydroxy 3 methylglutaryl CoA Elisa kit

Product Information

Cat. No.

E04H1417

Product Name

Rabbit 3 Hydroxy 3 methylglutaryl CoA Elisa kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

2.5-50ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

HMG-CoA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- HMG-CoA antibody and an HMG-CoA-HRP conjugate. The assay sample and buffer are incubated together with HMG-CoA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HMG-CoA concentration since HMG-CoA from samples and HMG-CoA-HRP conjugate compete for the anti-HMG-CoA antibody binding site. Since the number of sites is limited, as more sites are occupied by HMG-CoA from the sample, fewer sites are left to bind HMG-CoA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HMG-CoA concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit 3 Hydroxy 3 methylglutaryl CoA Elisa kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between HMG-CoA and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit 3 Hydroxy 3 methylglutaryl CoA Elisa kit

Summary of the Assay Procedure for Rabbit 3 Hydroxy 3 methylglutaryl CoA Elisa kit

Citations of Rabbit 3 Hydroxy 3 methylglutaryl CoA Elisa kit

E04H1417 has been referenced in the below publications:

To explore the inhibition of Polyinosinic acid combined statin on Cholesterol crystals as well as expression of hs-crp in atherosclerotic rabbits.

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