E04H0106 Rabbit Serotonin/5 Hydroxytryptamine ELISA kit
Rabbit Serotonin/5 Hydroxytryptamine ELISA kit is suitable for detecting samples from rabbit species. Serotonin/5 Hydroxytryptamine can also be called 5HT, Serotonin, N-Methyl-Gamma, 5 HT, 5-HT.
Product Information | |
Cat. No. | E04H0106 |
Product Name | Rabbit Serotonin/5 Hydroxytryptamine ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 25 ng/mL | 1 vial |
STANDARD C (0.5mL) | 50 ng/mL | 1 vial |
STANDARD D (0.5mL) | 100 ng/mL | 1 vial |
STANDARD E (0.5mL) | 250 ng/mL | 1 vial |
STANDARD F (0.5mL) | 500 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
5 HT ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- 5 HT antibody and an 5 HT-HRP conjugate. The assay sample and buffer are incubated together with 5 HT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 5 HT concentration since 5 HT from samples and 5 HT-HRP conjugate compete for the anti-5 HT antibody binding site. Since the number of sites is limited, as more sites are occupied by 5 HT from the sample, fewer sites are left to bind 5 HT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 5 HT concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-114.79% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-116 | |
1:4 | 91-109 | |
1:8 | 88-103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between 5 HT and analogues was observed. | |
E04H0106 has been referenced in the below publications:
经皮穴位电刺激对正畸家兔外周和中枢痛觉调控物质 5-羟色胺与P 物质的影响.
经皮穴位电剌激干预家兔正畸牙痛的作用及对脑脊液0-EP、5-HT影响的研究.
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