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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit Acetyl CoA acetyltransferase, mitochondrial ELISA kit (E04A1178)

E04A1178 Rabbit Acetyl CoA acetyltransferase, mitochondrial ELISA kit

Rabbit Acetyl CoA acetyltransferase, mitochondrial ELISA kit is suitable for detecting samples from rabbit species. Acetyl CoA acetyltransferase, mitochondrial can also be called acetoacetyl-CoA thiolase, ACAT1, ACAT, MAT, T2, THIL, acetyl-CoA acetyltransferase 1, Acetyl-Coenzyme A acetyltransferase 1.

Products

Specifications of Rabbit Acetyl CoA acetyltransferase, mitochondrial ELISA kit

Product Information

Cat. No.

E04A1178

Product Name

Rabbit Acetyl CoA acetyltransferase, mitochondrial ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ACAT1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- ACAT1 antibody and an ACAT1-HRP conjugate. The assay sample and buffer are incubated together with ACAT1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACAT1 concentration since ACAT1 from samples and ACAT1-HRP conjugate compete for the anti-ACAT1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACAT1 from the sample, fewer sites are left to bind ACAT1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACAT1 concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Acetyl CoA acetyltransferase, mitochondrial ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ACAT1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit Acetyl CoA acetyltransferase, mitochondrial ELISA kit

Summary of the Assay Procedure for Rabbit Acetyl CoA acetyltransferase, mitochondrial ELISA kit

Citations of Rabbit Acetyl CoA acetyltransferase, mitochondrial ELISA kit

E04A1178 has been referenced in the below publications:

To explore the inhibition of Polyinosinic acid combined statin on Cholesterol crystals as well as expression of hs-crp in atherosclerotic rabbits.

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