En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit Anti Endothelin 1 ELISA kit (E04E0177)

E04E0177 Rabbit Anti Endothelin 1 ELISA kit

Rabbit Anti Endothelin 1 ELISA kit ELISA kit is suitable for detecting samples from rabbit species. Anti Endothelin 1 can also be called EDN1, ARCND3, ET1, ET-1, HDLCQ7, QME, endothelin 1, PPET1, preproendothelin-1, Endothelin 1, anti ET 1, anti-ET1, anti ET1.

Products

Specifications of Rabbit Anti Endothelin 1 ELISA kit

Product Information

Cat. No.

E04E0177

Product Name

Rabbit Anti Endothelin 1 ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

5.0-100ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

anti ET 1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- anti ET 1 antibody and an anti ET 1-HRP conjugate. The assay sample and buffer are incubated together with anti ET 1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the anti ET 1 concentration since anti ET 1 from samples and anti ET 1-HRP conjugate compete for the anti-anti ET 1 antibody binding site. Since the number of sites is limited, as more sites are occupied by anti ET 1 from the sample, fewer sites are left to bind anti ET 1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The anti ET 1 concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Anti Endothelin 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between anti ET 1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit Anti Endothelin 1 ELISA kit

Summary of the Assay Procedure for Rabbit Anti Endothelin 1 ELISA kit

Citations of Rabbit Anti Endothelin 1 ELISA kit

E04E0177 has been referenced in the below publications:

化瘀解毒法对急性肺血栓栓塞症兔D-二聚体、内皮素-1、B型脑利钠肽表达影响.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.