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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit Brain Natriuretic Peptide ELISA kit (E04B0452)

E04B0452 Rabbit Brain Natriuretic Peptide ELISA kit

Rabbit Brain Natriuretic Peptide ELISA kit is suitable for detecting samples from rabbit species. Brain Natriuretic Peptide can also be called BNP 32, Brain natriuretic peptide 32, brain natriuretic peptide, Gamma brain natriuretic peptide, Natriuretic peptide brain type, Natriuretic peptide precursor B, Natriuretic peptides B, NPPB, NPPB protein, BNP.

Products

Specifications of Rabbit Brain Natriuretic Peptide ELISA kit

Product Information

Cat. No.

E04B0452

Product Name

Rabbit Brain Natriuretic Peptide ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

25-500 ug/mL

Sensitivity

1.0 ug/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ug/mL

1 vial

STANDARD B (0.5mL)

25 ug/mL

1 vial

STANDARD C (0.5mL)

50 ug/mL

1 vial

STANDARD D (0.5mL)

100 ug/mL

1 vial

STANDARD E (0.5mL)

250 ug/mL

1 vial

STANDARD F (0.5mL)

500 ug/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

BNP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- BNP antibody and an BNP-HRP conjugate. The assay sample and buffer are incubated together with BNP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BNP concentration since BNP from samples and BNP-HRP conjugate compete for the anti-BNP antibody binding site. Since the number of sites is limited, as more sites are occupied by BNP from the sample, fewer sites are left to bind BNP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BNP concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Brain Natriuretic Peptide ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between BNP and analogues was observed.


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Summary of the Assay Procedure for Rabbit Brain Natriuretic Peptide ELISA kit

Summary of the Assay Procedure for Rabbit Brain Natriuretic Peptide ELISA kit

Citations of Rabbit Brain Natriuretic Peptide ELISA kit

E04B0452 has been referenced in the below publications:

The effect of Xuebijing injection on B-type natriuretic peptide level of rabbits with acute pulmonary thromboembolism.

Effect of Galectin-3 Antagonist on Ventricular Remodeling and Myocardial Fibrosis in Rabbits with Ischemic Heart Failure.

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