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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit Collagen Type II Cleavage ELISA kit (E04C0059)

E04C0059 Rabbit Collagen Type II Cleavage ELISA kit

Rabbit Collagen Type II Cleavage ELISA kit is suitable for detecting samples from rabbit species. Collagen Type II Cleavage can also be called CIIC, C2C.

Products

Specifications of Rabbit Collagen Type II Cleavage ELISA kit

Product Information

Cat. No.

E04C0059

Product Name

Rabbit Collagen Type II Cleavage ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

10-250ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

10 ng/mL

1 vial

STANDARD C (0.5mL)

25 ng/mL

1 vial

STANDARD D (0.5mL)

50 ng/mL

1 vial

STANDARD E (0.5mL)

100 ng/mL

1 vial

STANDARD F (0.5mL)

250 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

C2C ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- C2C antibody and an C2C-HRP conjugate. The assay sample and buffer are incubated together with C2C-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C2C concentration since C2C from samples and C2C-HRP conjugate compete for the anti-C2C antibody binding site. Since the number of sites is limited, as more sites are occupied by C2C from the sample, fewer sites are left to bind C2C-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C2C concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Collagen Type II Cleavage ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between C2C and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit Collagen Type II Cleavage ELISA kit

Summary of the Assay Procedure for Rabbit Collagen Type II Cleavage ELISA kit

Citations of Rabbit Collagen Type II Cleavage ELISA kit

E04C0059 has been referenced in the below publications:

Integrated Trilayered Silk Fibroin Scaffold for Osteochondral Differentiation of Adipose-Derived Stem Cells.

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