E04C0059 Rabbit Collagen Type II Cleavage ELISA kit
Rabbit Collagen Type II Cleavage ELISA kit is suitable for detecting samples from rabbit species. Collagen Type II Cleavage can also be called CIIC, C2C.
Specifications of Rabbit Collagen Type II Cleavage ELISA kit
Product Information | |
Cat. No. | E04C0059 |
Product Name | Rabbit Collagen Type II Cleavage ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 10 ng/mL | 1 vial |
STANDARD C (0.5mL) | 25 ng/mL | 1 vial |
STANDARD D (0.5mL) | 50 ng/mL | 1 vial |
STANDARD E (0.5mL) | 100 ng/mL | 1 vial |
STANDARD F (0.5mL) | 250 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
C2C ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- C2C antibody and an C2C-HRP conjugate. The assay sample and buffer are incubated together with C2C-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C2C concentration since C2C from samples and C2C-HRP conjugate compete for the anti-C2C antibody binding site. Since the number of sites is limited, as more sites are occupied by C2C from the sample, fewer sites are left to bind C2C-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C2C concentration in each sample is interpolated from this standard curve. |
Quality Control on Rabbit Collagen Type II Cleavage ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-114.79% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-116 | |
1:4 | 91-109 | |
1:8 | 88-103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between C2C and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rabbit Collagen Type II Cleavage ELISA kit
Citations of Rabbit Collagen Type II Cleavage ELISA kit
E04C0059 has been referenced in the below publications:
Integrated Trilayered Silk Fibroin Scaffold for Osteochondral Differentiation of Adipose-Derived Stem Cells.
