E04C0029 Rabbit Corticotropin Releasing Factor ELISA kit
Rabbit Corticotropin Releasing Factor ELISA kit is suitable for detecting samples from rabbit species. Corticotropin Releasing Factor can also be called CRH, CRF, CRH1, corticotropin releasing hormone, corticoliberin, CRF.
Product Information | |
Cat. No. | E04C0029 |
Product Name | Rabbit Corticotropin Releasing Factor ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CRF ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- CRF antibody and an CRF-HRP conjugate. The assay sample and buffer are incubated together with CRF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CRF concentration since CRF from samples and CRF-HRP conjugate compete for the anti-CRF antibody binding site. Since the number of sites is limited, as more sites are occupied by CRF from the sample, fewer sites are left to bind CRF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CRF concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-114.79% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-116 | |
1:4 | 91-109 | |
1:8 | 88-103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CRF and analogues was observed. | |
E04C0029 has been referenced in the below publications:
脾肾阳虚型IBS-D大鼠模型探索及温肾健脾方干预CRF信号通路的机制研究.
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