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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit Cystatin C ELISA kit (E04C0868)

E04C0868 Rabbit Cystatin C ELISA kit

Rabbit Cystatin C ELISA kit is suitable for detecting samples from rabbit species. Cystatin C can also be called Cystatin 3, CST-3, Cystatin C, AD8, Amyloid angiopathy and cerebral hemorrhage, Cst3, CST3 protein, Cystatin3, CystatinC, Gamma trace, HCCAA, Neuroendocrine basic polypeptide, Post gamma globulin, ARMD11, Cys C.

Products

Specifications of Rabbit Cystatin C ELISA kit

Product Information

Cat. No.

E04C0868

Product Name

Rabbit Cystatin C ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

1.0-25ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Cys C ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- Cys C antibody and an Cys C-HRP conjugate. The assay sample and buffer are incubated together with Cys C-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Cys C concentration since Cys C from samples and Cys C-HRP conjugate compete for the anti-Cys C antibody binding site. Since the number of sites is limited, as more sites are occupied by Cys C from the sample, fewer sites are left to bind Cys C-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Cys C concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Cystatin C ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Cys C and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit Cystatin C ELISA kit

Summary of the Assay Procedure for Rabbit Cystatin C ELISA kit

Citations of Rabbit Cystatin C ELISA kit

E04C0868 has been referenced in the below publications:

Expression of cystatin C during orthodontic tooth movement in rabbits with the periodontal remodeling.

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